control or celatrol-treated cells. Features showcased by this model include. 304 signal level saturation. 340 to 740 nm (linear variable dichroic) 220 to 1000 nm for abs (spectrometer) Plate Formats: 6 to 384-well plates, LVis Plate with sixteen 2 µL microspots. When necessary, samples were. Accurate quantitation The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. The plate was read on a SpectraMax 340 PC plate reader (Molecular Devices, Sunnyvale, CA) at 450 nm, with reference at 650 nm. Stat Fax 3200 Microplate Reader. Alkaline phosphatase (ALP) staining assay ALP activity was detected using a SensoLyteVR pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Inc. 5, 2 mM pNPP, and recombinant enzymes, PTP1B activities were continuously monitored on a SpectraMax 340 Microplate Reader (SpectraMax, Silicon Valley, CA, USA) at 405 nm for 2 min at 30°C. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. The estimation of monodehydroascorbate reductase (MDHAR) (EC 1. The adapter is. , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. TDP-43 stress granular cells were treated with 70% ice-cold. ” SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Optical density was measured using a SpectraMAX 340 (Molecular Devices, Sunnyvale, CA). As a common indicator of equine T cell activation, cytokine induction. Molecular Devices Corporation 1311. The SpectraMax i3x reader provides the flexibility to add novel detection capabilities without the need for service engineers or costly downtime—it grows with you as your research areas expand. Application note showing how SpectraMax® i3x and SpectraMax®iD5 Multi-Mode Microplate Readers are used to performGq-coupled receptor assays using HTRF technology. The. spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software, metamorph software, spectramax i3 platform. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Analysis of ALA by Ehrlich’s Reagent Assay. Oxidative stress induction by H 2 O 2 and intracellular ROS analysis in L-929 cells. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Time-resolved fluorescence was measured on the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). , Sunnyvale, CA). 1 mg mL −1 CDDP loaded A-CHAM, B-CHAM, C-CHAM or D-CHAM. After the addition of sPLA2 (20 μ g), the reaction mixture was incubated for 40 min at 37°C, and the absorbance was read at 10 min intervals. Microplate Reader Shelf. Create custom fluorescence assays without having to purchase expensive filter sets. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. Awareness Technology, Inc. 30. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Products : SpectraMax Plus 384 reader,. 5 mm (bottom read) Beam diverges to focal spot near bottom of well. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. 6) and 0. ZERO BIAS - scores, article reviews, protocol conditions and moreAbsorbance was measured at 450 nm with ELISA Reader (SpectraMax 340, Molecular Device Co. On-site Repair Service available nationwide for Molecular Devices Spectramax 340 Plate Reader's. well, and strip-well microplates. SpectraMax Microplate Readers. 2. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. For more sample throughput, the system canThe optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). 5. Furthermore, the stain was extracted using 10% acetic acid, and its absorbance was measured at 595 nm in a microtiter plate reader (SpectraMax 340; Molecular Devices). Both readers feature an 8-channel read head for fast reads and. Call 1-800-745-2710. Microplate Readers. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deepThe SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. The ABS reader ranges from. 1: SpectraMax Plus 384 components. Changes in OD 405 nm were followed on the SpectraMAX 340 plate reader. SpectraMax Microplate Readers. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. Urinary proteins were measured using a Micro BCA assay kit following the manufacturer’s instructions (Thermo Fisher Scientific, Rockford, IL, USA). 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). 5 ng/ml; estradiol: 10 pg/ml. A microplate reader asThe absorbance was measured at 425 nm and read every 10 min using a Spectramax 340 plate reader (Molecular Devices ®, USA). The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. Reagena Poc Puumala® was used for detection of IgM antibody against PUUV in NE patient sera at the Department of Clinical Virology,. This is a used Molecular Devices SpectraMax 340PC Microplate Spectrophotometer. Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)Bob - 06 Apr. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. SpectraMax ® Plus 384 optics. Microplate Readers. The. Stat Fax 4700 Microstrip Reader. The centrifuged solution was transferred to a different microtitre plate and the optical density (OD) of the change in color was measured using a plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any . Table 1. Water – distilled water or equivalent. Irene Tieleman, Ido PenandSimon VerhulstSubsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. ). This was followed by an additional incubation at 37 °C for 30–60 min. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. reader (Molecular Devi ces). The plates were incubated for 4 additional hours at 37°C. LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurements in the visible range. We selected the response at t=10 h for statistical analysis, because at that time point the response variation was largest. Versteegh, Yoran H. Statistical analysis. 340 to 740 nm (linear variable dichroic) 220 to 1000 nm for abs (spectrometer) Plate Formats: 6 to 384-well plates, LVis Plate with sixteen 2 µL microspots. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. The absorbance was measured at 340 nm for 2 min at a 15 s time interval. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). , Fremont, CA, USA). Molecular Devices SPECTRAmax. Background: A computerized machine, SPECTRAmax 340, was used to evaluate the recently reported phenomenon of antagonism of the polyene amphotericin B (AMB) in Candida albicans pre-exposed to the triazole fluconazole (FLZ). The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed for every assay. NHDFs were seeded in microtiter plates (96 wells) at a density of 1x105 cells per well. The mixture was centrifuged for 10 min at 10,000 rpm, and BSA concentration in the supernatant was determined using bicinchoninic acid (BCA) assay. Assay buffer was 50 m M Hepes, pH 7. 305 reference level saturation. The values of IC 50 represent concentrations of compounds toA microplate reader (SpectraMax M5; Molecular Devices, Sunnyvale, CA) was used to measure the absorbance at 340 nm for the collected BPW (6) Stabilization protects islet integrity during respirometry in the Oroboros Oxygraph-2K analyzer Islets December 31, 2022 Justin J. Supplier: Molecular Devices. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. The absorbance was measured at 550 nm with a Spectramax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). MOLECULAR DEVICE M5. 003 OD Stray light ≤ 0. The optical density at 405 nm (OD 405) of the plates was measured with a microplate reader (SpectraMax 340 PC384, Molecular Devices) before and after a 1-h incubation at room temperature. Cytotoxicity was determined by measuring the release of LDH. PerkinElmer offers a variety of Microplates For Absorbance and Colorimetric Assays. Cross sections of mouse skin was prepared and stained as previously described . Acceptor filter: 665 nm. These models can no longer be repaired. plates can be used in the SPECTRAmax PLUS 384. The reduction of HED in the presence of NADPH and GR was recorded at 340 nm. LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). Or scan up to 384 wells at any time to determine the best assay conditions. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. SPECTRAMAX 340. CSF-1, IL-34 and calprotectin levels were analysed by ELISA according to instructions provided by the manufacturer (R&D Systems, Minneapolis, Minnesota, USA). Immunofluorescence and Aggregation Analysis. Binding affinity for denosumab. 5 g/L-EDTA 0. the SpectraMax iD3 is a fantastic plate reader for a. Centrifuge – Eppendorf 5417C with rotor FA 45–30–11. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). G. The protein concentration was determined spectrophotometrically at 280 nm in a 1 cm quartz cuvette using a SPECTRAmax-340 UV–vis spectrophotometer. AoSMCs were seeded into 6-well culture plates. 5. Created Date: 20051213090753Zmeasured using a SpectraMax 340 multiwell plate. The release of p-nitrophenol ( 400) 17000 M-1 cm-1) was monitored at 25 °C for the enzymatic hydrolysis of com-pounds 1-8. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The area under the curve (AUC) for the comparison of the growth rates was determined using. Briefly, cells were seeded into a. • TRF-based with 340/80 nm EX and 616/10 nm EM 0200-7027: EX range: 340/80 nm EM range: 616/10 nm: High fg levels of Streptavidin: High. Rapid, accurate DNA, RNA, and protein quantitation in a one-touch, full-spectrum micro-volume absorbance reader. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. TDP-43 stress granular cells were treated with 70% ice-cold. . SpectraMAX 340. 003 OD Stray light ≤ 0. 2 software was used to measure and calculate of the bioactivity activity studies. 340-850 nm: Wavelength Selection: Monochromator, turnable in 1 nm increments:. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance platereader (Molecular Devices, Union City, CA, United States). Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of assays. Proteolytic activity was determined using the synthetic chromogenic substrate Nα-benzoyl-DL-arginine p-nitroanilide (DL-BApNA), in 96-well plates, according to Ponce-Soto et al. Percent radical scavenging activity was determined by comparison with a DMSO containing control (shown in Table 3). 1 mM MnCl2 and 25 mM CHES buffer, pH 9. For more sample throughput, the system can The optical absorption was measured at 450 nm for BUN and 490 nm for creatinine with a spectrometer (SpectraMax 340). Molecular Devices SpectraMax M5e Microplate Reader. SpectraMax190,340PC384,Plus384,VersaMaxMicroplateSpectrophotometersUserGuide 4 0112-0126D SafetyInformation. Bertin Precellys Evolution Touch Homogenizer. High fg levels of Streptavidin High fg levels of Streptavidin Exceeds Cisbio certification requirements TRF-based with 340/80 nm EX and 616/10 nm EM AlphaScreen AlphaScreen and AlphaLisa detection using 1 W 680 nm. 10. 316 grating motor driver faulty. The absorbance was measured at 340 nm for 2 min at a 15 s time interval. Labsystems Luminoskan RT Microplate Reader. 5. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Values are expressed as the mean s ± SD of 3 separate experiments, each performed in triplicate. SpectraMax Plus 384 measuring instruments pdf manual download. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Multi-mode Microplate Readers. SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer to the Obsolete Microplate Reader Models article for additional information. A 0. Fully tested with WarrantyThe SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Description Control Panel Cuvette Chamber Back Panel Microplate Drawer The Control Panel TEMP on/off TEMP Figure 1. This reader only allows high performance HTRF measurement when assays are run in. Stat Fax 3200 Microplate Reader. The plate was covered and incubated for one hour at room temperature. These lab workhorses are used in drug discovery, research, bioassay validation, and. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. This is equivalent to having 511 built-in filters,Results: A total of 64% of the subjects reported improved quality of sleep during treatment with naloxone at a dose of 40 ng per 24 hours as compared with 9% with sham treatment (P=0. Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). Near bottom. In order to detect the Fc-tagged fusion proteins, goat anti-human IgG (Fc Fragment specific; Jackson ImmunoResearch Laboratories Inc, West Grove, PA, USA; Code Number 109-006-098;. The plate was covered and incubated for one hour at room temperature. TGFα release was calculated as described in the Results. 1 mL of acetylacetone. Although all samples initially were analyzed at the same volume, the volume of sample. 00. Cell permeabilization. Wound healing assay. Enzyme activities at lower concentrations were assayed by using a LS50B fluorometric. Unit powers. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. An absorbance was read at 490/630 nm using a spectraMAX 340 microtiter plate reader. Permeability of the monolayer was verified by adding 1% Evans Blue dye (Thermo Fisher Scientific, Inc. 0 2. 1 mL of acetylacetone. Molecular Devices SpectraMax Gemini XS Microplate Reader, 250-850nm, Molecular Devices, for detection of samples in microplates. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Cell Culture AO391 (derived from human aorta) and CD314 (derived from human carotid artery) cell lines were a generous gift from Wendy Bauer (Tufts Molecular Cardiology Research Institute) and were grown in DMEM (CellGro) supplemented with 10% FBS. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. Data were expressed as the mean percent viable cell vs. TheSpectraMax®Plus384addstheabilitytoread SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. 1% N-1-naphtylethylenediamine dihydrochloride, 2. 5), L-ascorbic acid (25. Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. On 1, 7, 14, and 21 days, the cells were washed with DPBS twice and incubated with CCK-8 solution for 2 h in the dark at 37 °C. Number of flashes. CD154 is an established indicator of antigen-reactive T helper cells in other species, but has not been characterized in horses. ), which was proportional to the number of damaged cells. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. After treatment, total cellular RNA was extracted with a cold RNA extraction solution (Ultraspec RNA;Call 1-800-745-2710. Percent Transmission Absorbance Competitor 0. SpectraMAX 340 microtiter plate reader. Finally, after another 45 min of decolorization, 100 µL of the solution was transferred to a new well and the amount of crystal-violet stain in the decolorizing solution was measured with a microtiter plate reader (SpectraMAX 340 Adjustable Microplate Reader; Molecular Devices Ltd. . With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. 0 3. 5. With SOFTmax PRO, the contents of the wells in a microplate can be mixed used in the SPECTRAmax PLUS. Confocal laser scanning microscopy (CLSM) Biofilms were formed on glass-bottomed Petri dishes (CELLview™; Greiner Bio-One GmbH, Frickenhausen, Germany) in the presence or absence (control biofilms) of the BIC 50 of antifungals as described above. Cell viability was determined using the blue formazan assay in which colorless 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) is metabolized to a blue product by mitochondrial dehydrogenases. 5. Description. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Male 8 pin mini Din to Female 9D. 6. For use with Spectramax 340 devices which have an 8-pin mini DIN serial socket. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating. BV-2 cells were treated with various concentrations of BT and/or LPS for 24 hIranian Journal of Basic Medical Sciences ijbms. Wavelength Range. 0% and ± 0. Molecular Devices Spectramax 340PC instructions. Clotting times (mean ± SD) for rSimukunin and rSV. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. MSD read buffer and TMB one component HRP substrate are from MSD and SurModics (Eden Prairile, MN), respectively. The Catalina. #ABS PLUS) SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. Finally, the absorbance at 450 nM was taken using a SpectraMax ® 340 ELISA plate reader (Molecular Devices, USA). 001/min at 425 nm. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideHigh-throughput assays for promiscuous inhibitors Brian Y Feng1,3, Anang Shelat1,3, Thompson N Doman2,RKipGuy1 & Brian K Shoichet1 High-throughput screening (HTS) searches large libraries of chemical compounds. SpectraMax 340 from Molecular Devices). Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 570nm on the indicated days (Cell Proliferation Kit I; Roche). Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of assays. SPECTRAmax® 190. The levels of rheumatoid factor (RF) antibodies were measured at the Clinical Immunology Laboratory of the Sahlgrenska University Hospital or Leiden Clinical Immunology Laboratory. 1% Chaps, 10 m M dithiothreitol, and 10% sucrose. Call 1-800-745-2710. 5. 312 gain calibration failed. CD154 detection in equine PBMC by an anti-human CD154 antibody (clone 5C8) was confirmed by Western blots and then applied for flow cytometry. Six wells were assayed for each set of conditions and stadard deviations. Call 1-800-745-2710. The manufacturer of the SpectraMax 340PC Microplate Spectrophotometer is Molecular Devices. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. . 5 mL sample or standard solution was well mixed with 0. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. 3. It measures the optical density (OD) of samples in both 96- and 384-well microplates at a selected wavelength for a single point in time (endpoint), over a specified period of time. Details. The reaction mixture was incubated for 5 min. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). Softmax PRO v5. chen@case. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. Effect of hypoxia on natriuretic peptides and VEGF production in AC16 cells. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. control. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. To. Quick View. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Introduction The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature. Products : SpectraMax Paradigm reader, SpectraMax Plus 384 reader, SpectraMax 250 (Obsolete), SpectraMax L reader, SpectraMax 340PC 384 (Obsolete), Gemini EM reader, Gemini XPS reader, SpectraMax 340 (Obsolete). Each experiment was performed in duplicate and repeated at. These robust readers have been placed in labs from Antarctica to the. 0 OD. Absorbance was measured at 450 nm with ELISA Reader (SpectraMax 340, Molecular Device Co. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross. Includes: Computer Loaded with Softmax Pro 5. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. Details. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Oxidative stress induction by H 2 O. 0. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. The cytotoxicity of the CDDP loaded CHAMs were done by treating the FaDu cells with DMEM supplemented with 0. The mixture changed to a red color by a reaction of LDH that was measured by a plate reader (SpectraMax 340 PC; Molecular Devices, San Jose, CA, USA) at a visible wavelength of. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. Call 1-800-745-2710. 97), and each plate contained at least six positive and six negative controls. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. Adhesion molecule expression To determine whether baicalein could modify the oxLDL-induced adhesion molecule expression, HUVECs were grown to confluence, pretreated with baicalein for 2 hrs and stimulated with oxLDL (150 μg/ml) for. 81. The cell growth was determined by measuring the optical density at 600 nm using a spectrophotometer (SpectraMax 340, Molecular Devices, USA). Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Then the samples were incubated for 1 h with human IL6 detecting antibody and then with HRP conjugate for 40 min. Quick View. 0 to 4. Wells were washed three times. Measurements of OD at 540 nm were then performed in a microtiter plate reader (SpectraMax 340 microplate reader; Molecular Devices Corp. The SpectraMax® 190 Absorbance Microplate Reader is the ideal equipment for many life science applications including DNA analysis. 0 1. 2 - 3. Wallac 1450 Microbeta TRILUX Liquid Scintillation & Luminescence Counter $1,000. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. Finally, after another 45 min of decolorization, 100 µL of the solution was transferred to a new well and the amount of crystal-violet stain in the decolorizing solution was measured with a microtiter plate reader (SpectraMAX 340 Adjustable Microplate Reader; Molecular Devices Ltd. The cell proliferation on different Ti discs was assessed by measuring the absorbance at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Devices, Sunnyvale, CA). The activity of generated thrombin was measured by the amidolysis of its substrate S-2238 and theThe purple formazan crystals formed were dissolved by adding 100 μL of DMSO to each well and absorbance was read at 570 nm in a spectrophotometer [SpectraMax 340]. 190–850 nm <± 1. 25–0. Other technologies such as imaging. Plates were read at a wavelength of 405 nm with a reference wavelength of 620 nm (Spectramax 340 PC). The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. 05 ng/mL; progesterone: 2. Following HRP-conjugated secondary antibodies, goat anti-rat IgG (Kirkegaard & Perry. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. Features demonstrated by the model include temperature control, a robot-compatible interface and data analysis software. The loading equaled the mass of BSA per mass of particles. The membranes were. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. 306 plate air cal fail,low light. P. The VersaMax and SpectraMax 190 read 96-well microplates. Gerritsma,B. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Photometric Performance SpectraMax 340PC384 Item Description Wavelength range 340 nm to 850 nm Wavelength selection Monochromator tunable in 1 nm increments Wavelength bandwidth. 4; 100 m M NaCl, 0. Amino Acid Analysis. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Read one sample or up to 384. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). Amino Acid Analysis. Details. SpectraMax Multi-Mode Microplate Readers. Assay setup for a 384-well low-volume plate. After washing, the dye was eluted with 3% HCl, and the absorbance measured with a SpectraMax 340 microtiter plates reader at 665 nm. Plate reader (SpectraMax 340 PC plate reader or comparable plate reader). When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Triton X-100 (see Note 2). Total Cell Count in BALF Cells collected from the lavage fluid were suspended in 1 mL of F-12 media supplemented with 10% FBS. DNA extraction from blood meal ELISA homogenate. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross awiderangeofconcentrations. Analytical Data Regression and Acceptance Limits The conversion of the optical density units for the test samples, VSs, and QCs to concentrations was performed using the Watson LIMS software (version. A. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. SpectraMax Multi-Mode Microplate Readers. Not all manufacturers' microplates are the same with regard to design, materials, orSpectraMaxABS Plus,SpectraMaxM2,SpectraMaxM2e,SpectraMax M3,SpectraMaxM4,SpectraMaxM5,SpectraMaxM5e,SpectraMaxPlus 384 0200-7200 Multi-Mode ValidationPlate. The optical density of each well was determined immediately using a microplate reader (SpectraMax-340, Molecular Devices) set to 450 nm. The possi-ble effect of zinc or copper on the binding of heme by A was tested as described above by using a 50 and 100 M final concentration of each metal. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. The optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). Male 8 pin mini Din to Female 9D. StakMax Microplate Handling Stacker. 340 nm / 70 nm. 306 plate air cal. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Page 2 1) All labor charges to repair the product for a period of one (1) year from the original date of. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. 1 mL of culture media with 10% FBS. 0 nm increments Wavelength Bandwidth: 2 nm Wavelength Accuracy: < ± 1. Perkin Elmer Wallac Victor2 1420 Multilabel Counter $1,500. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. A 0. Description 1. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. HTRF-compatible SpectraMax Multi-Mode Readers.